FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1986-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1986-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804832
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3700.037.225111
ATAGACG850.002748363434.100673
GCGAAAG4200.032.793552
CGAAAGC5150.028.141333
TCTATAC6450.024.716383
GTCCTAT6750.023.6266961
GTATAAG1550.00180948623.3841951
CTATCCT4154.5474735E-1122.698134
TACACCG1600.002182657822.6421595
TAATATA3254.0112354E-822.2952044
TATACAT4801.4551915E-1121.132685
CTATACC6650.020.7026924
CTATAAG1800.004309438620.1363891
GCGTGCG1850.005062393419.5836267
AATACTC3001.1110289E-519.321315
TATCCTA3802.0910193E-719.0670815
TATACCA6850.019.0392465
GTTCTAT8100.018.7939641
TAAGGGT6650.018.523464
TATTATA6401.8189894E-1218.1227512
Produced by FastQC (version 0.11.5)