FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1986-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1986-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGAATGGCAGGCAGAGGCAGAGGGAAAAGCAGGCTCCCTGCTGAGCA12.4390243902439024No Hit
TTCAGTAACGTTGGGCAAGTTGTTCTGTCCTCTTGCCACAGCTTCCATGT12.4390243902439024No Hit
GTGTTGAATTCAATCAAACTAAATCACTATCAACATTTTTACTGTTTATC12.4390243902439024No Hit
CAAAATACCAAACAATAAAAACAAACTTACTAAGCAAAAGCAATAATTCA12.4390243902439024No Hit
CTTAGAATAGTACCTATGAGAGTAAGCCCTCAAAAAAAGTTGTAAAAAAT12.4390243902439024No Hit
CCTTGCAGGTGGGTGTGTTTCTTGGTCTTAGGGGGAGGAGTGTTAAGGGT12.4390243902439024No Hit
GAAATAATGGAGAGGATTTTATGCATCAGCGAGGCTTTTTCATTTCATGG12.4390243902439024No Hit
NNNNNNNNNNNTNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12.4390243902439024No Hit
CAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGT12.4390243902439024No Hit
AACCAACAACACGAGGTTCCATCCAAAGATAAAGCTTACTCCTTCCATGA12.4390243902439024No Hit
GTACACAATCATTAATATTATAGTCAGTATTAAAAGAAATTAATTTATAT12.4390243902439024No Hit
CACCTGTGAATTCTACGAATCATCCACAGAAAAAAATGAATACCAATCCT12.4390243902439024No Hit
AATGGAAGCTGTCTGGTTGGAAAATGTAATTTGATTGTATGTGAAGCATT12.4390243902439024No Hit
GAGTCAGACACAAAAAGCCGTACATTGTATGAGTTCACTTACATGAAATT12.4390243902439024No Hit
GGCATACACTCCTGTCCCCTGTATGACCTATACAGTAGAAAAACAGCGGT12.4390243902439024No Hit
CCCCAAGCAGAATGAGAGACCTGTGCTGACAGATGCTGCCAGGAAGCTGC12.4390243902439024No Hit
GTTTTGACACCAAGTGTCAGCTGCCAGCAGTTGTTTTACTTATAACAGAA12.4390243902439024No Hit
CCCCATAACGTTTGTGGATATGGGAGCTTGGGAATCAAAGGAACTGACTT12.4390243902439024No Hit
TCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTT12.4390243902439024No Hit
GGATCATACCTCTTCCCGTATTGTAGCATTTGGTTTGCAGATACTTGGGT12.4390243902439024No Hit
ATGGAATGGTGCCATGAAGCTAGTCTTCATGGGAAAAAAAACAGAAGACT12.4390243902439024No Hit
CTGGCATCCCTTGGCATAAACACAACAGAACTGGGGAAAAAAGAATACAA12.4390243902439024No Hit
ATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCGGCCCTGCCCCGGCACCT12.4390243902439024No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC12.4390243902439024No Hit
GTGCATATTCTGCATGTTCACTAGCTTGCTTTTCATTTGAAATATCTTGA12.4390243902439024No Hit
GAGAGAGAACGCATGAGCCTGAGCCTAGCCCACCCCAGAAGATGTGACCT12.4390243902439024No Hit
GGGGATCGTAATCCCCACTGAGGAAGTAGTCTGGGCAGATGGAAGGCCTG12.4390243902439024No Hit
CTATCAACCTCACCAGATTCAATGCTCTCTGACCCTCCTTGGCCATAGCC12.4390243902439024No Hit
ATCTTGAACATTCCTGAGCCATTCAAGATATTTAACAGAATGGAAGCTGT12.4390243902439024No Hit
ACATACACAATCTTCTCTTTCTTTCTTTCTTTCTTTTTTTTTTTTCAAAA12.4390243902439024No Hit
GAGGGAGAAGATGACTCCCCACTGAGCAGGGAGCCTGACCTGGGGTTCGA12.4390243902439024No Hit
ACTCGAGACACACTGGGACAGGGAACAGCAGTGAAAGAAACAAAGCTGGG12.4390243902439024No Hit
AATACTCAATGCTCATATGAAATAAAACATAACAAATAAGGCTAATAACA12.4390243902439024No Hit
AGATGGAAGAATTCTCTTATCTGTGAGGGCTACTGTCATGTGACCACACA12.4390243902439024No Hit
GGCATAACCCACTGACCCCCCGGGCACTACAATATCCCCTCCCCGTGCAT12.4390243902439024No Hit
GTCCTGGAATTGAGCCCTGCATGAGCTCCCTGGTCAGCACGGGGAGCCCC12.4390243902439024No Hit
GGGGCAGTAAATATTTATAAATGTTGGATAATTGTACTGCATTAATTCCA12.4390243902439024No Hit
GAACAGAGAGGTCTTATTTGGGGCAAGAATGTGTCTTTAAAAAAGGGAGG12.4390243902439024No Hit
CCCTAGAATTGCTTTTTAGCAAGTTCCCCAGGAAATTACACTACACAAGA12.4390243902439024No Hit
CAATAATTTATTGACATTTCAGACTAAATTTCAACATGTGAAGTTGACCA12.4390243902439024No Hit
ATTCTAAACAAGCTGATCAGCTCTCCTTTCCTGAAAGGACAGAATACATT12.4390243902439024No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)