FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1985_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1985_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718962
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2150.047.191781
ACACGTT855.3275573E-542.6639145
CGAAAGC3050.033.261713
GCGAAAG3150.032.2102662
TCTATAC6700.029.2015113
GAGTGCG1050.00772362327.6068787
CTATACC6400.026.0413934
TTCTATA9550.025.0430582
ATACCAC8650.022.6169386
GTATATA3955.9117156E-1022.0171431
GTTCTAT9600.021.8929161
GTCTTAC1700.00309861921.3156191
CATAATG3457.5264325E-821.0066952
TATACCA9050.020.8166545
ATAGGTC2104.3273796E-420.7037183
GATATAG1800.004315171420.1314141
TTAGAGT5800.019.9897964
GAGTATA6900.019.971651145
GTCTAAG2608.0963226E-519.5119881
TACCACT9150.019.0080157
Produced by FastQC (version 0.11.5)