FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1985-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1985-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889072
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG450.00965955748.3070037
AGCGAAA4150.040.167881
GTCCTAT7500.033.8225171
GCGAAAG5200.032.049842
CGAAAGC5500.031.6191273
GTAATAC3709.094947E-1225.4590953
TACTATA2601.1504926E-725.0824832
TACCGCA1500.001498890824.1535027
CCTATAT1851.8372148E-423.5007023
TACACCG1851.8372148E-423.5007025
ACCGTGC1550.001814072323.3743558
TATACTG3503.5215635E-922.77335
GTACCTA4951.8189894E-1221.957736
TAGTACC3655.7189027E-921.837414
CTATATA2455.1346797E-520.7030014
TATATCT6700.020.54854
TGTAATA5650.020.5197892
ACTATAG2155.0741294E-420.2215353
GTATAAC2608.0958685E-519.512991
TCCTATA2608.109892E-519.5085982
Produced by FastQC (version 0.11.5)