FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1985-d10-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1985-d10-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743222
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9530.1282254830992624No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8990.12095982088797157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGT350.003581652262.0748149
ATATTCG450.00968254548.277166
TTAACGC800.002045903636.2151834
GTTGACA7950.035.546251
TAACGCA850.002757208234.077995
AGCGAAA1751.0135409E-733.1244161
ACATGGT8850.031.9120185
GACATGG9150.031.6635514
ACCGTGC1153.1829538E-431.4851028
CGTCTAT950.00471600830.540209145
GCGTGCG950.00475191430.492899
GCGAAAG2151.8189894E-830.3319511
GTATAAG2451.9826984E-929.575371
CGAAAGC2502.4610927E-928.9819132
TGACATG10100.027.9738163
GGTCCTA12700.027.9418139
TACGGTG1050.00775105527.5869455
TTTAACG1500.001500525624.1483383
TGGTCCT14400.024.138588
TTGACAT11900.023.7456842
Produced by FastQC (version 0.11.5)