FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1985-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1985-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847797
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9490.11193717363944436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1000.072.449859
GTACTAC1209.572617E-636.2441941
TGCCGCA1701.0343066E-425.5765782
GCATGTT8900.024.4198554
TAGAGTG5100.024.1413945
GCGAAAG3503.5051926E-922.7820641
TAGCATG9650.022.5285912
ATAACCG1952.6328568E-422.2843655
TTAGAGT4607.2759576E-1222.0486554
TAGACTG6650.020.6926255
TAAGACC4301.715307E-920.217374
ATGTTGT13300.020.151656
GTAGCAT12300.020.037441
GGTTATA3651.3618228E-719.8598331
GTAGGCT3301.1457778E-619.7578834
GTCCTAT11050.019.6801051
GTATAAT2608.08538E-519.5161061
AGCATGT11150.019.4932353
AGCGAAA3351.3159406E-619.4744911
TAGTACC3051.28284755E-519.0021174
Produced by FastQC (version 0.11.5)