FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1985-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1985-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011670
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC15220.1504443148457501No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA14330.14164697974635998No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG13610.13453003449741516No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA12830.12682001047772495No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC12750.12602923878339775No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG10460.10339339903328161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2300.050.423951
ATCGAAT855.3264637E-542.667477145
TTACGTG2850.040.6829382
TACTGCG1354.9953087E-737.5696377
GTTGACA9500.036.623711
CCGACTA3200.036.2260674
TACGTGT3350.034.605723
GTGATCG1551.4720335E-632.7219439
CGTGTTG3700.031.3291025
AGCGAAA3750.030.9266871
TATACTG4550.030.2532425
GTACTAG2202.2690074E-829.6527211
ACATGGT12050.028.8591735
GTTATGT7800.028.8079131
CATCGTT1306.5005454E-427.8717252
ACGTGTT4250.027.2760984
GCTATAT3057.1304385E-1026.1419241
TAGTCGG2259.15632E-725.7620358
TACTTAG6500.025.6381787
AGTCGGC2301.1099801E-625.1994975
Produced by FastQC (version 0.11.5)