FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1985-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1985-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771912
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGT1051.8586116E-434.5070658
CATAGCG1301.6597898E-533.4453127
TACCGTC900.00363634532.2065937
TAACGAC900.003642130932.196143
AGCGAAA3850.032.0036961
CTACGGA950.004745385630.5016063
TAGTACC2651.4006218E-1030.0719324
CCTATAC2202.280649E-829.6350863
GTCCGTA1000.0060822228.9915851
GCGAAAG4250.028.9859352
TACGGAC1255.175264E-428.9784094
ACATCCG1100.00969312226.3508518
TAGCGTG1450.001229659524.9878759
TATTCCG1450.001234322124.9716595
CGAAAGC5000.024.6300493
ACCGTGC2651.3819408E-724.6106998
GTGTAGC4151.8189894E-1224.4507331
GTCTAAC3601.8553692E-1024.1596531
GTGTAGG5400.022.8174481
CTATACT2953.8998587E-722.1021754
Produced by FastQC (version 0.11.5)