FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1984-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1984-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14772
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC210.1421608448415922No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG200.13539128080151638No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC180.12185215272136475No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT160.1083130246412131No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT150.10154346060113728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATC100.0070202085145.02
TTGGAGT100.0070202085145.09
CTACTCT100.0070202085145.0145
TAGATAC100.0070202085145.03
GATATTG300.001897758972.500012
GGATATT300.001897758972.500011
ATATTGT300.001897758972.500013
ATACATA350.00349677862.1428576
GTTCTAT400.00593302554.3751
TGGAGAT400.00593302554.375145
TTCTATA400.00593302554.3752
CTATACC450.00945207348.3333324
TATTGTG450.00945207348.3333324
TCTATAC450.00945207348.3333323
ACATATT450.00945207348.3333328
GTGGGCC450.00945207348.3333328
ATTGTGG450.00945207348.3333325
TACCACT450.00945207348.3333327
Produced by FastQC (version 0.11.5)