FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1984-d10-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1984-d10-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033008
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC26410.25566113718383593No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG23790.2302983132754054No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG23680.22923346188993698No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT19120.1850905317286991No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA13980.1353329306259003No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG13820.13378405588340073No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA12310.119166550501061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG7900.063.267675
ACTGATC9650.051.7992748
GTACTGA10000.050.703376
AGCGAAA2500.049.288131
AGGTACT10850.048.741074
TAGCTCG450.00967243948.2912645
CAGGTAC12000.044.0764543
GACAGTA9400.042.3873987
CTGATCC12200.041.5681959
GCAGGTA12300.041.248232
GTAGAAA13100.038.175982
AGCAGGT13700.037.563951
CAGTATG10500.035.8787239
ACAGTAT12050.034.869278
TTGGACA11700.034.674014
GCGAAAG3600.034.226212
GGATAGC11150.033.1325725
GGTCGTT2450.032.525847
CCGGTAA1352.1330234E-532.242603145
AAGGTCG3150.032.1988569
Produced by FastQC (version 0.11.5)