FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1984-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1984-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632475
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC12320.194790307917309No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT10980.17360369975097828No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8870.1402426973398158No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC8670.13708051701648288No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA8460.13376022767698328No Hit
AAGCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGAC8180.12933317522431717No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT7960.12585477686865093No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC6690.10577493181548679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1353.6379788E-1253.6644259
AGCGAAA2050.053.0309681
GTCTACG657.3466654E-444.6004031
GCTACTA1652.910383E-1143.9072579
GCGAAAG2650.041.017462
GTTACGT951.0304963E-438.145081
CGAAAGC2900.037.4755363
GGGTACG800.002039407136.2378271
TTACGTG1153.1699653E-431.5061632
GTTATGT4000.027.1783711
GTATATA3503.6379788E-1226.9195291
GTTGACA5200.026.481491
GTCTTAC1100.00968478126.3547821
AGTGATC3057.1486284E-1026.1284188
CCTATGC2507.870767E-826.0829753
CTATGCG1400.001003324925.8739194
TATGCGG1450.001231751624.9797385
TATACTG3201.2496457E-924.9016765
GCAGTGC5250.024.8487951
CGTGTTG1751.2619383E-424.8369965
Produced by FastQC (version 0.11.5)