FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1983-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1983-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907004
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1007.221388E-1057.9736441
CTATACG553.2357566E-452.6713184
TTACGTG1255.2259566E-946.3686752
GTGATCG1755.4569682E-1141.3868949
CTACGTC700.001062698941.380045
CGTGTTG2156.1865285E-726.9451435
TACGTGT2301.1126413E-625.192023
GTATATA6100.023.7596911
ACGTGTT2653.8142243E-621.8635674
GCGAAAG3706.682967E-921.544261
AGCGAAA3106.2699473E-721.0388241
GCTATAC1750.003679866420.6934473
TGTACCG2857.169041E-620.3270385
TATACTG5750.020.150285
GTAGCAT12350.019.9504451
TGTTGGC10300.018.9846867
TAACCAC4654.5602064E-918.6877615
CGAAAGC4003.5879748E-718.1127622
CTATACA2801.4375352E-418.1057644
GGTTAAT3252.211222E-517.8380451
Produced by FastQC (version 0.11.5)