FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1983-d10-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1983-d10-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812376
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG13690.168518026135681No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10060.12383428363220972No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC8960.11029375560085478No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC8930.10992446847272691No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG8690.10697017144770402No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA8520.1048775443883128No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA8290.10204634307266587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGT259.405319E-486.955238
GTTCGTA400.006037721454.40401145
AGCGAAA2200.046.126831
GTTGACA9750.038.6586761
TACAACG951.0333412E-438.1288575
GCGAAAG2750.036.8969152
ACATGGT10450.035.3558545
GCTACTA1656.0450475E-835.131269
GTTACGT1301.6567388E-533.4546241
CAGGTCG5400.032.205647
TAATCGT900.003638085232.2036559
AGGTCGG5450.031.9101758
CGAAAGC3200.031.6965683
GACATGG11950.030.9197834
TTGACAT12800.029.443412
GAATCGC1000.006079924828.9940071
CGAATTA1000.006089085728.9850777
CTACTGA13100.028.2037146
GGTCCTA14300.027.8685479
TGACATG13300.027.781313