FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1983-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1983-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068616
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC17380.1626402748976246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3800.034.329731
GTAATAT3200.033.972131
GCGAAAG4450.030.9409982
GTATAAA5800.029.989191
TATACTG5650.028.2040565
GTGATCG1958.80532E-626.006519
CAACCCG3357.4578566E-1125.985147145
CGAAAGC5050.025.8213793
GTTGACA10300.023.2197821
GTAGCAT12750.022.1684781
GCAGTGC9200.022.0572641
CTATACA2653.8091348E-621.8676324
TCTATAC16050.021.213933
TATATAC4451.1459633E-1021.1632163
TATACAT5500.021.0714595
TAATATA5201.8189894E-1220.9039652
GTTATAG2806.1099236E-620.7068211
TAGATCC1750.003674510620.699069
TTGACAT11250.020.612862
ACATGGT11300.020.5120415
Produced by FastQC (version 0.11.5)