FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1982_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1982_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734689
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9490.1291703019917271No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8930.12154802916608251No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGT300.001943002372.429678
TAGTCGG951.0345183E-438.1208848
AGCGAAA2008.980351E-932.6111221
GCGAAAG2151.8226274E-830.3255962
GTTACGT1204.0671276E-430.1954841
TAGCGTG1050.00774372227.5922589
CGAAAGC2456.4857886E-826.6104433
GTATATA3900.026.0145721
TAGCATG12550.025.9761072
GTAGCAT14600.025.314571
GTATAAC1801.5241391E-424.156391
GCATGTT12650.024.0494354
GTTATGT5950.023.1414151
CCGACTA2202.243354E-523.0473774
GTTGACA8500.023.0196151
TCAGGCG1902.2068524E-422.872539
ATGTTGT16450.022.4554026
CATTACC3554.1600288E-922.4460563
CATGTTG11950.022.4259245
TTGTTTA16900.021.8574779
Produced by FastQC (version 0.11.5)