FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1982_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1982_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847385
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12570.1483387126276722No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC11230.13252535742313115No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA9500.1121096077933879No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG9120.10762522348165239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG4050.048.2853475
TATACGG450.00967652748.2853475
GTTACGT1553.4742698E-837.4131431
GTATAGC1553.4906407E-837.393253
TAGTCGG1153.174658E-431.4997488
CTACTGA12400.029.2031216
TACTGAA12500.028.9797677
GACTTAA3700.027.4278811
GTCTATA2405.2781616E-827.1829871
ATACTGT5800.026.2223876
TAGCATG10000.026.0894992
CGTGTTG1958.8201505E-625.9998045
GACTATA2259.1367474E-725.7674082
CATTCTA11050.024.9220942
TTACGTG2351.3347035E-624.6709212
GTAGCAT14150.024.5895581
ATTATAC2951.4379111E-824.5591263
GGTTATA3851.6370905E-1124.4764581
TTATACT3851.6370905E-1124.4619944
GCATGTT10700.023.6970084
Produced by FastQC (version 0.11.5)