FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1982_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1982_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792041
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1451.943772E-839.960589
AGCGAAA3450.035.7074851
TATACTG3000.033.795725
GCGAAAG3700.033.2863962
CCGACTA1652.404011E-630.7272624
CGAAAGC4550.027.0595073
TTAGAGT4353.6379788E-1223.3103374
GTATATC1902.199748E-422.8837431
CTATACA2303.1649488E-522.0434724
GGCGCGC2353.7362246E-521.5744638
GTCCTAC2704.4614426E-621.4711671
GTATAGC2404.3968113E-521.123663
GTTGACA6950.020.8532921
CTACTGA9400.020.8000016
CTATAGC2104.3270306E-420.7043421
GTTATGT4201.2751116E-920.7043421
TTAGACA4652.0736479E-1020.2488424
GTATTAT8950.020.2416731
GTATTAG2556.9808564E-519.8924051
GTCCTAT10600.019.8253821
Produced by FastQC (version 0.11.5)