FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1982_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1982_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850024
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12880.1515251334079979No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT10390.12223184286561321No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA10040.11811431206648283No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9830.11564379358700459No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA8620.10140890139572531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1350.059.0517461
TTACGTG1700.046.8829842
TACGTCG657.36671E-444.5775039
ACGTGTT1953.6379788E-1240.8603064
TATACTG3850.037.623715
AGCGAAA3100.037.405211
ATACGTC1001.3982409E-436.2192238
GCGAAAG3300.035.1299482
CGTGTTG2302.7284841E-1134.6383555
TCGCCAT850.00273337234.138954145
CGAAAGC3950.031.1742043
CCGACTA1902.0925472E-730.4986024
GTTGACA7900.030.2733651
TAGTCGG1255.178872E-428.975388
GCTACTA1504.423481E-528.9753789
AATACGT1306.514409E-427.860947
ACCTCTA4750.027.450367
GCACCGT7100.026.523636
CTTACCG1100.00964138826.3801145
GTTATGT4400.026.353671
Produced by FastQC (version 0.11.5)