FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1982-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1982-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720173
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1350.059.0466841
TTACCGT700.001059943541.4007266
TAGCGGG750.001488017338.640685
GCGAAAG2404.3655746E-1133.2114522
TATTATC2455.638867E-1132.5336652
CGAAAGC2455.638867E-1132.5314033
TATACTG3255.0931703E-1126.751245
GCATAGT1100.009701556526.3459176
TAGCTAT1958.795811E-626.0081466
TACTACT2259.155083E-725.760454
TAGAGCG1701.03513055E-425.5728139
TATAGCT3501.3096724E-1024.8404374
GTATAAC2101.5598713E-524.1554621
TCTATAC5750.022.6819743
GTCTTAA3203.3989636E-822.6457441
ATAGCTA2252.6640071E-522.5403945
CCTTTAG3055.359725E-721.3835221
TATACAG2505.9992777E-520.2877663
ATACACG1800.00432096620.1267533
GTCTAGG4051.8897481E-819.6822261
Produced by FastQC (version 0.11.5)