FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1982-d10-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1982-d10-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861436
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG12900.1497499524050539No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG10330.11991604715846564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3650.057.585291
GCAGGTA6350.043.3651162
GCGAAAG5300.041.018152
CGAAAGC5200.040.4086953
GTACTGA6300.040.246856
GCGTTGG750.001488880438.6369746
GTATGGA6200.038.5770231
ACTGATC8400.037.9492348
GGTACTG7550.034.5455
AGGTACT7700.032.933074
TGCGTTG1102.4460416E-432.9311525
CAAGGCG900.00364058732.1993529
TACACCG1801.3001227E-732.1993525
GGATAGC6700.031.3583245
TATGGAT7200.031.2003782
ACACCGT2502.4665496E-928.9777326
CTGATCC10400.027.8648249
CCGTATA1050.007690816727.631577145
CAGGTAC10600.027.342253
ACCGTGC1606.8601206E-527.1682038
Produced by FastQC (version 0.11.5)