FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1982-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1982-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820838
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGG907.512589E-540.249233
CGAAAGC5400.034.882663
TGATCCG2103.8016879E-1034.4972349
CTACGGT1102.4468047E-432.9291764
TAAACCG2257.9853635E-1032.1954545
CATAGCG950.004743225430.5046773
CCCTACG1204.0713933E-430.1906032
TACGGTG1255.178164E-428.975915
TATATAC4050.028.6216743
TATACTG2603.750756E-927.8614525
TTGCTCG1050.007736671327.5977889
GCGAAAG6750.026.8360922
GTATTAC1958.7753015E-626.0167141
AAACGTT1958.804442E-626.0056066
TACACCG1400.001004162825.8713475
TAATACT6650.025.0558854
AGCGAAA7300.024.8202531
ATATAGG1801.5234997E-424.1583791
TTAACGC2401.6073845E-624.1495363
GCGATAG1500.00150129224.1465915
Produced by FastQC (version 0.11.5)