FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1982-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1982-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982023
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA350.00357792462.0926369
AGCGAAA6250.042.9093741
GCGAAAG6700.040.0192382
CGAAAGC7300.037.7149963
TTACGTT1854.2145984E-935.2417684
GAGCGCG1851.6552622E-731.3260179
TACGTTC2054.0694977E-728.268385
GTCGTGC1358.12086E-426.8301538
GTCCTAT6350.026.2533421
GCACCGT3057.185008E-1026.1224176
AGCACCG2001.0725667E-525.3532035
TATTACG2051.2947547E-524.7449232
ACCGTGT2351.3397967E-624.6609068
ACGTTCC2101.5666486E-524.1434446
ATTATAC3304.7222784E-821.95533
GTATAGG3706.668415E-921.5487171
GTATTAC4058.076313E-1021.4761621
TATAGTA2404.3917673E-521.1276665
CCGGTAA1750.00364340920.729242145
TATAGGT3157.313138E-720.7049332
Produced by FastQC (version 0.11.5)