FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1982-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1982-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716233
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10710.14953234492127562No Hit
ATTTTAGAGTGATCGCTTACACTGAAAAATATTTTGGCCTAAGTTTCATA8380.11700103178714188No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG2150.067.408749
AGCGAAA2300.050.413541
GCGAAAG2800.041.4082262
CGAAAGC3150.036.8073123
TTAGAGT4500.032.2041444
TAGAGTG4900.031.0496545
GTATAAA4250.030.6929511
AGTGATC4800.030.1956128
GTAATAT2802.6375346E-1028.4701481
GAGTGAT5100.028.41947
GTCCTAT7500.028.0215261
ATCGTCC1751.252863E-424.864067145
GGACCGT1801.5254904E-424.1531096
GTATTAT5000.023.190231
TCTATAC10900.022.6035733
GTACTGC1952.6211925E-422.2982961
ATATTAC2603.215724E-622.2967383
ATTAGAC4058.094503E-1021.4709323
GTATTAG1700.003099504621.314551
GTTCTAT10550.021.2943461
Produced by FastQC (version 0.11.5)