FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1981_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1981_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080355
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16680.1543936946651795No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12110.11209278431626642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1855.84145E-627.417451
AGCGAAA5900.027.0191351
CCGACTA2756.8230293E-926.3431344
TTACGTG1958.798856E-626.0090162
GCGAAAG6200.025.7093752
ATATAGC1450.001232081124.9805583
TACGTGT2051.2981913E-524.7368453
GTATACC1600.002182177722.6438771
TATACTG3854.3291948E-1022.579835
GTTGACA9550.022.0036831
GTCTAAC2003.114739E-421.7381211
CGAAAGC7650.020.8334583
CTATATA4551.546141E-1020.7029721
ACATGGT10750.020.2168245
GTTATGT5750.020.1628951
TAGTACC5800.019.9844469
TATACGG1850.00506988719.5793575
CTACATC4904.1836756E-1019.2197363
CCACCGA4152.5062036E-819.2063711
TTATACG1900.005914705319.0641084
Produced by FastQC (version 0.11.5)