FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1981-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1981-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854484
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14950.17495939069660754No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT11020.12896672143656288No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC10690.1251047415750324No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT8740.10228395148417058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1206.002665E-1154.346481
TTACGTG1402.746674E-1046.5799672
TACGTGT1704.0017767E-1142.6097153
TATACTG4200.041.389875
ACCTCTA4500.033.815597
GATATAC2258.0035534E-1032.1940083
ACATGGT12500.031.87025
GTTGACA12900.031.456361
GCTACTA2101.4466423E-831.0551329
ATTACCT4750.030.4995844
CTACATC4350.029.973733
CATTACC4400.029.6331213
CCATTAC4550.028.6645982
TACTGAA17300.028.0633097
CTACTGA16850.027.9379586
CGTGTTG2354.3351974E-827.7400175
TTGACAT14650.027.6971532
TGACATG15550.027.0181223
AGCGAAA3255.0931703E-1126.7551921
TTGATTA7100.026.566502145
Produced by FastQC (version 0.11.5)