FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1981-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1981-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652003
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT8200.12576629248638427No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC7730.11855773669753053No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC7210.1105823132715647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1300.078.009689
ACCGTAC350.00356835562.1322561
GTTACGT805.8774276E-754.3657261
TTAGAGT2950.046.6618654
GTGCGAA803.748853E-545.280434
TTACGTG1053.832609E-641.411962
AAGGTCG1157.1812065E-637.793519
GAGTGAT2750.036.8773047
TAGAGTG3400.036.221565
TATACTG3900.035.2928055
GCGGACC1051.8624144E-434.4940769
ACGTCCG900.003642646832.194479
GGTACTG2755.456968E-1231.6115445
GTTACAT2807.2759576E-1231.0518193
GCGAAAG3050.030.896371
GGGTAGC950.004730040730.521111
TATAACG950.00474427530.5023695
CTATGCG1453.5054458E-529.9787674
GTTATGT3151.8189894E-1229.915531
GGCGGAC1255.1779364E-428.9750258
Produced by FastQC (version 0.11.5)