FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1981-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1981-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708641
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGTA406.640172E-572.54768145
CGCATAA450.00961297348.365124145
TATACGG1203.6452548E-948.293375
CGTCTAG855.3192216E-542.67511145
TACGGGG1502.615161E-838.637437
GCGAAAG5150.038.0024451
GATAGCC800.002043788836.2225847
ATATACG1656.052687E-835.1249354
CGAAAGC5450.033.2458232
GACCGGG1204.0748794E-430.185497
ATAGCGG1000.00609786528.9760255
CCTATAT4000.027.1823041
TATAATA5100.027.0008262
TATACAG2756.7811925E-926.3548722
GTATACA3201.2423698E-924.917111
AGCGAAA4151.8189894E-1224.4531561
GATACTA4303.6379788E-1223.600141
TAGTATG4053.274181E-1123.2572943
GGTAGTA4103.8198777E-1122.9834141
TACGGAG2852.7986425E-722.8758075
Produced by FastQC (version 0.11.5)