FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1981-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1981-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509581
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC7570.14855341937788105No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT259.405424E-486.9502647
TCGTTAT450.00962080948.35319145
TATCGTG450.00965823248.3057028
GTATAAC1002.7385468E-643.483671
TTATACT2950.039.2918364
GTATATA1502.6038833E-838.6521531
CGCAATG800.002031575236.264896145
TATACTG3350.034.5968785
GTCTATA850.00274577534.1048431
GTTATAC850.00274577534.1048431
TCGGACC900.003640240532.197484
GCGAAAG2951.4551915E-1129.4804571
GTATTAG1504.4057822E-528.9891151
GCTACTA1000.00608868128.9834219
TATAACA1306.500241E-427.8686752
GACTACG1050.0077190827.608681
CGAAAGC3153.45608E-1127.6032562
AGGTCGT1606.857432E-527.1666226
GTATAGT1100.00968491426.3537431
AACGATC1100.00969419326.3485647
Produced by FastQC (version 0.11.5)