FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1980_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1980_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTGAGCAGCTCGGTTTCTTGGGGAGAGGAGCTTTGAAATAGGAAGGT16.25No Hit
GACTCAACAGTCTGCTGAGTCTCAGAGTTGGAGGGGGAAAGACTCTGAAG16.25No Hit
ACTCTATTCTCTTTGTCTTTATAGGTAAAAAATTAGTAGGCCGTCCGTAC16.25No Hit
CACACACTCCTTAGCAAACACCCCATCCGCGCCCGTCTGGAATTATGGGC16.25No Hit
AATAATACAATGAAACAAAATTCACAGAATGCAGTAAAACAGGTTATAAA16.25No Hit
GATTCTAAGTCCAGGTAGGAACTTGAAAACAAGCGGAAATTCTTACTCCC16.25No Hit
CCCTCACACCACAAGACAAAAACACACATCAGAGCGCCCCTTACTGAAAA16.25No Hit
CACTGAGACTGGGTGGGAGAGAAAAGAGAGAGGGAAAAAACCCTAAAAGC16.25No Hit
GAGGCAAAGTCCCAGCCCTGCAAAAATCCATGCCCCAGAGGCCAGCAGGC16.25No Hit
CTGTTAAATGGTGTTGCACAACAATTGTCCCAGACTGCCCATTGCTGAGC16.25No Hit
GCGCTGACATGAGTGACAATACCGGCGTTGAGCTTTCGAAACCCACAACA16.25No Hit
GAACCCGGACTGTGACCAGAGGGTGAATAAAGGGGACAGAAGCAAAGACC16.25No Hit
CTAATGAGACCACTAAAAAAGCCACGGTACCCTGTACTCCGGTATGAGAT16.25No Hit
CATAAGACTTTTAATCTGGCATTCTAACTGATATAGCTTATGAGAGATAA16.25No Hit
GCCTGAGAGGCCCCCAGGGGTCGGTGGATCTGACCGTGGCAAGCGCTGCC16.25No Hit
ATTTAGAGAATATAAGACTAAAATCTTAAGATTTTGCCATCACATTCTCC16.25No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)