FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1980_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1980_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGAAATGTTTGCAATTAGACCTTAAAAAAAATTTTTTTTTTATTATTTG18.333333333333332No Hit
CATTGATGCTTAATGCTGGTACATATATTTTGTATTCTTTGCCGAGGATT18.333333333333332No Hit
GTCTTTACCCAAGAGATGTCAACTGGGAGATCAAAAAATGTCTCCAGACA18.333333333333332No Hit
GTACAAGAGAACTAGCGGTAGACTAGTTGAAGAGAAATATATTTTTTTTC18.333333333333332No Hit
GTTTAGTTTCTGTGTTTATGAATATCCAGTTTTCTTCTTGTAATTGATTT18.333333333333332No Hit
CACTGTGACCTGACGTTACTCAGACAGTCCTCACTCCAGTCACATGTGGT18.333333333333332No Hit
CCCCGCTGTATCCCATCCGGTCCCCAAAATGCACCCCGGACTCTTAGAGG18.333333333333332No Hit
GATTATACTAGATTATCCATGTGGGACCAATCTAATCACATCAAACTTTA18.333333333333332No Hit
AGAGGGAACATGACCAGGGGGAGGGGTAGAGGGAAAGAGGGCCTCTAAAG18.333333333333332No Hit
TTCCAACTCTGGGCCAGAATCCACCAGTGGGTCTCAAAGTCAATATAAAT18.333333333333332No Hit
GTTTTAGAGTTGCAGAGAACTTGGGAGAAATGCCGCATCAATCCTCCAGT18.333333333333332No Hit
ACTCCCGGAGTAATGCTTTTTTGCTTACCTTAGGCGGGAAGAACACTGCA18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)