FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1980-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1980-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919059
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11350.12349588002511264No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG10120.11011262606644406No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC10080.10967739829543044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1050.075.934831
TATACTG3450.050.4093975
TTACGTG1851.8189894E-1243.0911062
GCGATTA907.463334E-540.294815145
GTAATAT3600.040.268471
GACGACT800.002041566236.231764
TACGTGT2353.45608E-1133.919093
CCGACTA2755.456968E-1231.6204414
CTACTGA14800.030.3563356
GTCGGTT1204.0684777E-430.1947757
AGACGAC1204.069776E-430.1931293
TACTGAA14450.030.0902947
TAGTCGG1453.5007986E-529.9865348
GTTGACA8850.029.484711
ACATGGT9050.028.8252645
GGGATAC3900.027.896408145
TCGGTTC1306.499581E-427.8720998
CGAAAGT1050.007727087527.6051464
ACGTGTT2903.9835868E-1027.4861584
CGTGTTG2903.9835868E-1027.4861585
Produced by FastQC (version 0.11.5)