FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1980-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1980-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581168
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT7960.13696555901219612No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC7470.12853426203782728No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG6770.1164895520744432No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC6350.10926272609641273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT259.4116264E-486.9373864
AGCGAAA2900.039.995341
GCGAAAG3000.038.652162
CGAAAGC3550.032.6525423
GTATTAG1453.4906683E-529.9965041
TATAACC1453.5050776E-529.978413
GCACCGT6800.027.693486
GTATAAG2104.98545E-727.6158281
ATTATAC1606.856801E-527.1679343
ACCGTGT7350.025.6255938
GTATATA2559.482574E-825.5852551
AGCACCG7050.024.6567155
TAGGACT4103.8198777E-1122.9712624
CCGTGTT7900.022.9245539
CCCTATA1902.1933563E-422.8920691
GTATTAT3854.274625E-1022.5947691
CACCGTG8350.022.5566627
ATAGCAC9000.022.5393243
GTATGAC2252.6638392E-522.5393243
CATTACC5800.022.4838073
Produced by FastQC (version 0.11.5)