FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1980-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1980-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032503
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC36820.3566091333390799No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG23110.22382501552053602No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC22620.2190792665977726No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA22200.21501148180683252No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG21570.2089098046204224No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC20090.19457570583330025No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT19650.19031421700469636No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT19430.18818347259039442No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA19180.18576217211959672No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC16320.15806249473367148No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT16140.15631915839469715No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC15190.14711821660566604No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG14500.1404354273062645No Hit
CTATAGGACTTGCAAGCTAGTTGGAATCAACATGAGCAAAAAGAAGTCTT14350.13898264702378588No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG13980.13539912232700535No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA13790.13355893396919913No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA13530.13104078147956955No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT13510.13084707744190574No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG13240.12823207293344427No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT12960.12552021640615088No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG12530.12135557959637888No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC12300.11912798316324506No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC11850.11476964231580924No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC11650.1128326019391711No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA11370.11012074541187775No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC11360.11002389339304583No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA10900.10556870052677812No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT10880.10537499648911432No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA10580.10246943592415712No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC10500.10169461977350186No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT10480.10150091573583805No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA10430.10101665564167853No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT10380.10053239554751899No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT10340.10014498747219136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA650.0100.3050843
ACGAATA650.0100.300225
TATGGCG301.5942223E-596.73081145
GGTACTG1450.094.920135
AGTAGAA3250.089.194671
GCGAAAG1050.082.803542
GCAGGTA1850.082.244062
CGAAAGC1100.079.0282443
GATAACG903.6379788E-1272.470671
AGGTACT2200.065.853684
GAAACGC451.18996315E-464.4872145
GCTTATA451.197705E-464.4027562
GACAGTA3700.058.742917
AACACTG5900.055.250125
CTTATAT553.2316698E-452.6854973
TTTGGAC4300.048.8566133
CGCAATT604.929001E-448.365406145
TATAGAC604.9621443E-448.2997257
GGTCAAA1700.046.8927881
AGAAACA6400.045.2744034
Produced by FastQC (version 0.11.5)