FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1980-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1980-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749907
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG15660.20882589441090696No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC12180.16242014009737207No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC10160.13548346661652713No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT8520.11361408814693022No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA7780.10374619786186821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTC450.00962005848.35638145
TCGCCAT5100.038.400658145
GTATAAT1102.4421388E-432.939421
TATAGCG1102.4440553E-432.935022
GCGAAAG2455.638867E-1132.531742
CTATAGG4500.028.986691
TATACTA2553.0468073E-928.4088365
GTCGGTA4600.028.383095145
TAGGACT6450.028.07854
GCTACTA1306.498831E-427.871829
CGAAAGC2954.8748916E-1027.0124683
TTATACT5100.026.9883944
AACACTG10800.026.8305665
GTACCGA4950.026.349787
TATACTG6050.026.342745
CAACACT10000.026.0793114
GGATAGC2507.885683E-826.0793115
TAACTAT2808.229108E-925.8809741
CTAACCG2259.16556E-725.7573434
TACCGAG5650.025.6502257
Produced by FastQC (version 0.11.5)