FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1979-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1979-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206866
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCT450.00968656848.25979
TATAGCG450.00968656848.25977
GCGAAAG1109.970245E-846.099542
TAGTACC657.387027E-444.5366364
TACACCG855.3825504E-542.5717855
GGAGTCG700.001062635341.3654568
GAATAGT951.0294252E-438.1366731
TAGGACT951.0367911E-438.090554
AGCGAAA1354.967533E-737.5716821
AGTACCT1001.4021288E-436.1860165
CGAAAGC1301.6685146E-533.4024773
TAATACT1102.4548278E-432.896384
TATTATG1352.1457156E-532.1965032
GAGTCGA900.003648765232.1731349
CTAGTAT900.003653092332.165353
CTAATAC1153.1863333E-431.4661033
ATAGTAC1204.0891304E-430.1550143
TATACTG1204.0891304E-430.1550145
CATTATA1504.4059627E-528.9768522
TACTGTG1753.8004346E-628.955827