FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1979-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1979-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930508
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG350.00357687862.0969623
CGACGTA350.003579166362.0869375
TTACGCT450.00967120848.292444
GTGATCG1251.2723471E-534.7705549
GCGAAAG3850.033.882012
GTTACGT950.00473300930.5185531
AGCGAAA4150.029.6912381
CGAAAGC5150.026.7278183
TAGTCGG1100.00970867726.3427478
TATACTG4550.023.8795915
GTCCTAT10150.022.1372261
TACTGAA12650.021.760237
GTCCTAG5301.8189894E-1220.5136491
CTACTGA12100.020.3535696
GTATTAT10800.020.1337661
GTATATA6900.019.958691
ATTATAG5505.456968E-1219.7581233
ACACGAC1850.00503204719.60439145
CTATATA4503.0067895E-919.3252932
CATTCTA9000.019.3252932
Produced by FastQC (version 0.11.5)