FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1979-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1979-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071979
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTACG1000.006089778628.9852332
AGCGAAA4850.028.3902531
GCGAAAG5100.026.9985731
CGAAAGC5700.025.4208933
TACACCG1600.002187035522.6351955
TATACTG5200.022.286965
GTCCATA4102.1773303E-819.4431321
GTATTAT13200.018.1174641
ATACCCG2050.00917581517.6664926
ATATACC4557.1990144E-817.5152743
GTATATA8050.017.1046871
GTTATAT6455.2750693E-1116.8534551
CTAGTAC2600.001820300116.7191263
TAGTCGG2600.001820300116.7191268
TATACCC6104.311005E-1016.623885
GCCTATA3504.1768653E-516.5645391
ATTAGAC4901.6757076E-716.2641833
CTTAAGC5352.5680492E-816.2503663
CTATTAT4501.2279488E-616.1044121
TCCTATG11800.015.9664422
Produced by FastQC (version 0.11.5)