FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1979-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1979-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871783
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9540.10943090195610605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1950.059.4604459
GTTACGT1651.369699E-939.5322271
TTACGTG2101.4448233E-831.0592522
TCGCCAT1204.045085E-430.224283145
ACCGTGC1703.0230422E-629.8378098
TAGAGTG5100.029.8309525
GCGAAAG4350.028.3238561
AGCGAAA3600.028.1850131
CCGACTA1555.5208046E-528.048684
TTAGAGT5250.027.6034624
AACCGCT1358.10583E-426.8382427
GAGTGAT4950.026.3502737
CGAAAGC4650.024.9364592
AGTGATC5400.024.1544198
CGTGTTG3203.410787E-822.6395635
GAGCGAA2252.657891E-522.548011
TATACTG5001.8189894E-1221.733985
GTACCTA4801.4551915E-1121.1302596
TACGTGT3106.2760046E-721.036513
TAGTACC4501.3278623E-1020.9326254
Produced by FastQC (version 0.11.5)