FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1978_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1978_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4300
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA90.20930232558139536No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT90.20930232558139536No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC50.11627906976744186No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA50.11627906976744186No Hit
CTCCAGGACCCCCCATTTCTGTAAATCACAGAATCTGGGCTTGAAGGGTG50.11627906976744186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA100.0068489052145.09
CTTAGGT100.0068489052145.05
CACCTTA100.0068489052145.02
TTAGGTC100.0068489052145.06
AGGTCAT100.0068489052145.08
ACCTTAG100.0068489052145.03
CCTTAGG100.0068489052145.04
TAGGTCA100.0068489052145.07
AAAAAAA900.00488613411.27777965-69
Produced by FastQC (version 0.11.5)