FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1978_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1978_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8500
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC250.29411764705882354No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG250.29411764705882354No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA240.2823529411764706No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC200.2352941176470588No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC170.2No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT170.2No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG150.17647058823529413No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA140.16470588235294117No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT110.12941176470588237No Hit
CCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATCCCTATCTGCA110.12941176470588237No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG100.1176470588235294No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA100.1176470588235294No Hit
ATTATGTACTCAGTGGCCCTGCAATGAGACACTTCCGCTGTGAAGTAGTT90.10588235294117646No Hit
CTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGTTC90.10588235294117646No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG90.10588235294117646No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC90.10588235294117646No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG90.10588235294117646No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG90.10588235294117646No Hit
CAATATCACTAATAACTATACTACTAGTAGTAACAGCAAGCAATGCAGAT90.10588235294117646No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT90.10588235294117646No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT90.10588235294117646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCT100.006968524144.999987
TCCTAAG100.006968524144.999981
GGGTAAC100.006968524144.99998145
TAACGGA100.006968524144.999984
ACCGTAC100.006968524144.999988
CTAAGAC100.006968524144.999983
TAAGACC100.006968524144.999984
ATAACGG100.006968524144.999983
CCGTACT100.006968524144.999989
AAGACCG100.006968524144.999985
GACCGTA100.006968524144.999987
AACGGAG100.006968524144.999985
TCATAAC100.006968524144.999981
ACGGAGC100.006968524144.999986
CATAACG100.006968524144.999982
ATAGGTG203.7341096E-4108.749993
GTATAGG203.7341096E-4108.749991
TATAGGT203.7341096E-4108.749992
CTTCCTG203.7341096E-4108.749999
CTGCTTG259.0675376E-487.0145
Produced by FastQC (version 0.11.5)