FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1978-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1978-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536448
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC553.2305202E-452.6846664
CGAAAGC2450.038.4454733
TACACCG1553.4928235E-837.3856285
ATATTCG800.002038257436.2409931
GTAGTAC850.002749223334.096443
CGTAGTA900.003619549632.23528145
CCTATTG1402.754243E-531.0550122
ACCGTGC1902.0882908E-730.5016488
GCGAAAG3151.8189894E-1229.9048232
GTATATC1907.1611285E-626.7038881
TCGTGCG1100.00964857826.374317145
ATTATAG2207.515755E-726.3472483
CTATAGT2207.528015E-726.3423334
GTATATA4400.024.7097681
GTATATG3952.3646862E-1123.854831
AGCGAAA3702.6011548E-1023.507671
ACACCGT2852.798024E-722.8741026
TAGTACC1952.6268786E-422.2896654
TAATACC3655.7225407E-921.8316574
TATTACA4801.4551915E-1121.134662
Produced by FastQC (version 0.11.5)