FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1978-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1978-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873581
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3153.45608E-1127.6156851
TATACTG5550.026.1034875
GTATCTA1450.001227188324.9969561
CCGTACC1500.001489198224.18036145
GTAATAC3301.7880666E-924.147113
AGCGAAA3402.5065674E-923.4530261
GTCCTAT7900.022.9402451
ATAGTAT2653.8037379E-621.8703338
GAGCGAA2404.3661552E-521.1432571
TCCTATG10050.020.1895982
GTATTAA11600.019.372641
TCTATAC8300.019.2013153
TTATACT5707.2759576E-1219.0635054
CAGTGTA5356.548362E-1118.9576939
ATACAGC4603.969035E-918.8977366
GTCTAAT3902.7340138E-718.5874791
CGAAAGC4908.649295E-917.7468342
ATACACC4155.285401E-717.4557424
TATGGAC4205.9917693E-717.2479343
TCTACAC4205.9917693E-717.2479343
Produced by FastQC (version 0.11.5)