FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1978-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1978-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335680
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4250.049.444682
CGAAAGC5250.038.6391753
TAGACGA1453.5091478E-529.9775479
CTCGTAC1100.00970631126.3448933
TGCCGTA2351.3392655E-624.6633057
GTCTATA3851.6370905E-1124.4722881
TGCACCG4002.7284841E-1123.5413365
CTAGACG1902.2042925E-422.8784588
GTATTAA22950.022.7374521
AGACTCG1952.631328E-422.2876555
ATGACGA5250.022.112688145
CCGTACA2953.9044426E-722.102099
CCGGACG2303.1630843E-522.047054
ACACGTC2104.3359323E-420.6995588
ACGAGAT1400.00133362320.6995587
ACGGGTC2455.1452273E-520.6987829
GTTATAC5002.5465852E-1120.2855663
CGGACGC2506.017885E-520.2817655
TAAACCG1800.004330280220.1207985
TATAGAC5204.3655746E-1119.509012
Produced by FastQC (version 0.11.5)