FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1978-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1978-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824940
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATGGGTGTGTGTAGACCCTTAAAAAATGTCACAGGCCCTGTGTGTC11860.14376803161442042No Hit
GGGCTGAACCGTGCACAGTGTCTTATGGGTTGAGATTGGCTAACTTATAA9680.11734186728731787No Hit
GTGTAGACCCTTAAAAAATGTCACAGGCCCTGTGTGTCGCGAATATGTTG9440.11443256479259098No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8520.10328023856280458No Hit
GTTATAGAGCCTACTAATCCCCAAGGCAAAGAACTGGACTGTGATGCCCT8450.10243169200184256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4250.054.58161
GCGAAAG4450.052.1095162
AGACGGT2550.051.19824145
CGAAAGC5250.044.1663363
CTATATA4600.039.3830382
TATATAC5850.038.397813
ATATACG951.0320483E-438.1370474
GTATAAG1051.854756E-434.5196151
GAACCGT4800.033.2009356
TATACGG1751.0147596E-733.120735
ACCGTGC6700.031.3577428
GCTATAT4400.031.3030151
GCGTATC1402.759795E-531.04888
ATAACAG5400.030.8627593
GAATAAC5500.030.31451
AACCGTG5750.030.2388327
CTAGTAG1000.006090081328.9841563
ATATACC5300.028.710724
GTTATAG5950.025.5851271
ACACCGT3751.0913936E-1125.111986
Produced by FastQC (version 0.11.5)