FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1977-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1977-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217448
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12270.10078459203185679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2150.040.4429441
GCGAAAG5900.038.0660822
AGCGAAA6750.034.351541
CGAAAGC6650.033.7659653
GTCGAAG2051.1492375E-831.8000493
TATACTG7150.031.3982495
AGTCGAA1851.6544254E-731.3290142
TTACGTG3204.1836756E-1127.168132
TACAGCC9200.026.7688837
GTTGACA11400.026.6958921
GAGCGAA3005.9117156E-1026.5687661
GTGATCG2207.5484604E-726.3394329
GACGACT1100.00971968226.3372674
TATACGG1100.00972553126.3340175
CGTGTTG3306.366463E-1126.3340155
GCACCGT6500.025.6271326
AGCACCG6500.025.6250255
ACATGGT11950.025.4525435
TGACATG13000.025.0731163
AAGTCGA2051.296054E-524.7425351
Produced by FastQC (version 0.11.5)