FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1977-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1977-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904236
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA11860.13116044926324544No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11620.1285062749105322No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC11120.1229767450090463No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG9120.10085862540310273No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9050.1000844912168947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAA100.0070947106144.97291
ATCGATA350.003572950662.1140373
CGAATTA450.00965440148.3135957
TCGATAT450.0096607448.305574
ATCGACA855.3295145E-542.662727145
GGTCGTT700.001058711841.4116557
GTTACGT1553.475361E-837.412361
GTTGACA8950.032.396181
TATACTG4650.031.161435
TTACGTG1652.3916327E-630.7484232
GACGACT1204.0714192E-430.190984
CAATTCG1453.4997658E-529.987759
TACACCG1703.0274132E-629.8324895
GACTCGC1000.006079696628.9945811
ACATGGT10400.028.5621475
TCGCTCA1050.00772970427.6031824
CTACTGA13950.027.528986
TAGCATG12400.026.8871562
GTGATCG1358.1014034E-426.8408879
GGTTATA3850.026.3587111
Produced by FastQC (version 0.11.5)