FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1977-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1977-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023755
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10490.10246592202235888No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT10290.10051232961011179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1450.054.968729
ATCGTAG450.00964969848.3200151
GTTACGT1700.046.898841
GGTCGTT1851.8189894E-1243.0814827
GGTACTG4500.038.6390085
TTACGTG2201.6370905E-1136.2329252
AAGGTCG2252.0008883E-1135.419095
GGTCGAA850.002745720634.1082461
GTCGTTT2353.45608E-1133.916878
AGGTCGT2353.45608E-1133.9118966
GTTGACA10100.033.0107041
AGGTACT6000.032.6032564
GTTATGT6050.032.346461
TATACTG9500.032.0297055
CGTGTTG2601.10958354E-1030.6511365
GACGATG2451.9645086E-929.602503145
GCAGGTA5650.029.499372
AGCGAAA6300.028.7619151
AGCACCG6550.028.758045
ACATGGT11350.028.7239325
Produced by FastQC (version 0.11.5)