FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1976-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1976-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403204
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAC100.007101936144.913652
CGAAAGC2350.061.6577223
GCGAAAG2400.060.380692
AGCGAAA2500.057.979871
TATAGGC1406.633836E-736.223917
GTCTAAC1504.4026674E-528.9899331
GTACAAA2603.7198333E-927.8749351
GGCCCGT1050.007730177627.5991738
CGGTCTA1050.007734865427.5957459
CTGTGCG1050.007734865427.5957459
GATTAGA1358.090664E-426.8425311
GTATAGG1358.11419E-426.8291956
TATGTCG1100.00969882426.3446667
AGTATAG1701.034844E-425.569825
TAATATA3251.4915713E-924.5208034
CTCCATA2701.6532613E-724.1522752
TAGTATA1500.001498996624.1492754
TAGGGCT2451.9193376E-623.6564334
GATATAC1851.8323271E-423.505351
TATTACA2502.2853947E-623.1861842
Produced by FastQC (version 0.11.5)