FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1976-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1976-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901131
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGT657.3613797E-444.5843471
GCGAAAG3600.044.2747342
TCGTCTA750.001490015238.631189
AATGTCG1157.1870472E-637.791375
CGAAAGC4550.035.024723
TATTACG900.003644388432.192659
AAGGTCG1153.178921E-431.492815
CGTGTAC1000.00609979128.9749953
AGCGAAA5650.028.210451
GTATTAG2853.255991E-1027.9629881
TGTCGTC1050.00774476127.5921657
GTCGGTC1358.125543E-426.8272088
GTCCTAT8400.025.8748441
AGCCGGT1550.001818622123.3643347
TAGAGCG1902.2066094E-422.8737265
ATGTCGT2353.7366066E-521.5747266
CCTATAT4801.4551915E-1121.12763
TATAAGC3851.0591975E-820.6998752
TGTATAC3508.8135494E-820.6964263
GCGTTCC2455.1495557E-520.6952749
Produced by FastQC (version 0.11.5)