FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1976-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1976-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874489
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3900.029.7182222
CTACTAG2001.0747237E-525.3461029
GTAATAT3001.7156708E-824.1543521
TGTAATA5750.023.9360892
ACTATAC2751.9897925E-723.700253
GTATACT2202.2471098E-523.043234
TATACTA3905.0931703E-1022.2810125
CGAAAGC5250.022.0700743
CCATACG3004.6037894E-721.725239
AGCGAAA5400.021.4705331
TACCGAG1750.003682599420.6906957
CTATACT5353.6379788E-1220.3051154
ATAATAC7350.019.7054253
TATACTG7750.019.6216645
GTATAAG1850.00506126719.5846081
ATACTAT3351.329905E-619.4532016
AGGTACT4102.1927917E-819.430184
CTATAAC2256.890314E-419.3234831
TATACCG2659.4342344E-519.1280365
GGATAGC3051.2878649E-518.9936495
Produced by FastQC (version 0.11.5)